Human Nephronectin (NPNT) ELISA Kit | KTE62216

Human Nephronectin (NPNT) ELISA Kit | KTE62216 | Gentaur UK, US & Europe Distribution

Application: This Human Nephronectin (NPNT) ELISA Kit employs a two-site sandwich ELISA to quantitate NPNT in samples. An antibody specific for NPNT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNPNT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NPNT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NPNT bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Nephronectin microplate
• Human Nephronectin standard
• Human Nephronectin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Nephronectin (NPNT) ELISA Kit has high sensitivity and excellent specificity for detection of Human NPNT. No significant cross-reactivity or interference between Human NPNT and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Nephronectin contains 561 amino acids. In situ hybridization detected nephronectin in the developing mouse kidney and in several other tissues and organs. Expression appeared particularly prominent at epithelial-mesenchymal interfaces in tissues undergoing morphogenesis. The deduced 565- and 582-amino acid proteins have calculated molecular masses of 61.9 and 64.0 kD, respectively. Both proteins contain an N-terminal signal sequence, followed by 5 EGF domains, and a C-terminal MAM domain. They also contain an RGD integrin-binding site, a potential glycosylation site, and a potential tyrosine phosphorylation site. EGF repeats 2, 4, and 5 contain Ca (2+) -binding EGF domains. In the longer variant, the 17 additional amino acids are inserted N-terminal to the first EGF domain.

Alternative Names: NPNT; EGFL6L; POEM;

Search name: NPNT; EGFL6L; POEM;

Tag: NPNT