Human Neurogenic locus notch homolog protein 1 (NOTCH1) ELISA Kit | AE58200HU

Human Neurogenic locus notch homolog protein 1 (NOTCH1) ELISA Kit | AE58200HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: NOTCH1

Alternative Name: TAN1; hN1; neurogenic locus notch homolog protein 1|notch1|translocation-associated notch protein TAN-1

Application: ELISA

Range: 0.156-10 ng/mL

Sensitivity: 0.055 ng/mL

Intra-Assay: ≤6.8%

Inter-Assay: ≤11.3%

Recovery: 1, 02

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate NOTCH1 in samples. An antibody specific for NOTCH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNOTCH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NOTCH1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NOTCH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: NOTCH1 encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .