Human NHE1 (Sodium/Hydrogen Exchanger 1) ELISA Kit | ELK3886

Human NHE1 (Sodium/Hydrogen Exchanger 1) ELISA Kit | ELK3886 | Gentaur UK, US & Europe Distribution

Alternative Names: SLC9A1; APNH; Solute Carrier Family 9 Member 1, Sodium/Hydrogen Exchanger; Antiporter, Na+/H+, Amiloride Sensitive; Na (+) /H (+) antiporter, amiloride-sensitive

Species: Human

Assay Type: Sandwich

Sensitivity: 0.054 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sodium/Hydrogen Exchanger 1 (NHE1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sodium/Hydrogen Exchanger 1 (NHE1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sodium/Hydrogen Exchanger 1 (NHE1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sodium/Hydrogen Exchanger 1 (NHE1) in the samples is then determined by comparing the OD of the samples to the standard curve.