Human NKB (Neurokinin B) ELISA Kit | ELK1801

Human NKB (Neurokinin B) ELISA Kit | ELK1801 | Gentaur UK, US & Europe Distribution

Alternative Names: NK-B; TAC3; NKNB; ZNEUROK1; Neuromedin K; Tachykinin 3; Neurokinin Beta

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 3.12 pg/mL

Standard: 800 pg/mL

Detection Range: 12.5-800 pg/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Lenght: 2h

Research Area: Neuro science;Hormone metabolism

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Neurokinin B (NKB) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Neurokinin B (NKB) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neurokinin B (NKB) in the samples is then determined by comparing the OD of the samples to the standard curve.