Human NLRC4 (NLR family CARD domain-containing protein 4) ELISA Kit | E-EL-H5599

Human NLRC4 (NLR family CARD domain-containing protein 4) ELISA Kit | E-EL-H5599 | Gentaur UK, US & Europe Distribution

Type: Sandwich

Assay Time: 3.5h

Sensitivity: 46.88pg/mL

Detection Range: 78.13~5000pg/mL

UniProt ID: Q9NPP4

Target Name: NLRC4

Target Synonym: N/A

Test Principale: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NLRC4. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NLRC4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NLRC4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human NLRC4. You can calculate the concentration of Human NLRC4 in the samples by comparing the OD of the samples to the standard curve.

Specifivity: This kit recognizes Human NLRC4 in samples. No significant cross-reactivity or interference between Human NLRC4 and analogues was observed.

Research Areas: Cancer, Cell Biology, Epigenetics and Nuclear Signaling, Metabolism, Immunology

Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.