Human NPHN (Nephrin) CLIA Kit | E-CL-H1175

Human NPHN (Nephrin) CLIA Kit | E-CL-H1175 | Gentaur UK, US & Europe Distribution

Type: Sandwich

Assay Time: 3.5h

Sensitivity: 18.75pg/mL

Detection Range: 31.25~2000pg/mL

UniProt ID: N/A

Target Name: NPHN

Target Synonym: NPHS1, CNF, Nephrosis 1, Congenital Finnish Type, renal glomerulus-specific cell adhesion receptor

Test Principale: This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NPHN. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NPHN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NPHN, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human NPHN. You can calculate the concentration of Human NPHN in the samples by comparing the RLU value of the samples to the standard curve.

Specifivity: This kit recognizes Human NPHN in samples. No significant cross-reactivity or interference between Human NPHN and analogues was observed.

Research Areas: Cancer, Signal transduction

Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.