Human NRP1 (Neuropilin 1) ELISA Kit | ELK2885

Human NRP1 (Neuropilin 1) ELISA Kit | ELK2885 | Gentaur UK, US & Europe Distribution

Alternative Names: CD304; BDCA4; VEGF165R; Vascular endothelial cell growth factor 165 receptor

Species: Human

Assay Type: Sandwich

Sensitivity: 26.2 pg/mL

Standard: 4000 pg/mL

Detection Range: 62.5-4000 pg/mL

Sample Type: serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Neuro science;Developmental science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuropilin 1 (NRP1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Neuropilin 1 (NRP1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neuropilin 1 (NRP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neuropilin 1 (NRP1) in the samples is then determined by comparing the OD of the samples to the standard curve.