Human NT-ProANP (N-Terminal Pro-Atrial Natriuretic Peptide) ELISA Kit | ELK2399

Human NT-ProANP (N-Terminal Pro-Atrial Natriuretic Peptide) ELISA Kit | ELK2399 | Gentaur UK, US & Europe Distribution

Alternative Names: NT-Pro-ANP; N-ANP

Species: Human

Assay Type: Sandwich

Sensitivity: 14 pg/mL

Standard: 2500 pg/mL

Detection Range: 39.07-2500 pg/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Endocrinology;Cardiovascular biology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-Terminal Pro-Atrial Natriuretic Peptide (NT-ProANP) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to N-Terminal Pro-Atrial Natriuretic Peptide (NT-ProANP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain N-Terminal Pro-Atrial Natriuretic Peptide (NT-ProANP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of N-Terminal Pro-Atrial Natriuretic Peptide (NT-ProANP) in the samples is then determined by comparing the OD of the samples to the standard curve.