Human Nucleoside diphosphate kinase A (NME1) ELISA Kit | AE30868HU

Human Nucleoside diphosphate kinase A (NME1) ELISA Kit | AE30868HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: NME1

Alternative Name: AWD; GAAD; NB; NBS; NDPK-A; NDPKA; NM23; NM23-H1; NDP kinase A|OTTHUMP00000174772

Application: ELISA

Range: 0.625-40 ng/mL

Sensitivity: 0.31 ng/mL

Intra-Assay: ≤5.3%

Inter-Assay: ≤6.9%

Recovery: 0, 97

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate NME1 in samples. An antibody specific for NME1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNME1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NME1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NME1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: N/A

Stability: N/A