Human OCT4 (Octamer Binding Transcription Factor 4) ELISA Kit | ELK5047

Human OCT4 (Octamer Binding Transcription Factor 4) ELISA Kit | ELK5047 | Gentaur UK, US & Europe Distribution

Alternative Names: POU5F1; OCT3; OTF3; OTF4; Octamer-4; POU Domain Class 5 Transcription Factor 1; POU Class 5 Homoeobox 1; Octamer-binding protein 3

Species: Human

Assay Type: Sandwich

Sensitivity: 0.061 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction;Developmental science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Octamer Binding Transcription Factor 4 (OCT4) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Octamer Binding Transcription Factor 4 (OCT4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Octamer Binding Transcription Factor 4 (OCT4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Octamer Binding Transcription Factor 4 (OCT4) in the samples is then determined by comparing the OD of the samples to the standard curve.