Human Octamer transcription factor 2B (OTF2B) ELISA Kit | KTE62566

Human Octamer transcription factor 2B (OTF2B) ELISA Kit | KTE62566 | Gentaur UK, US & Europe Distribution

Application: This Human Octamer transcription factor 2B (OTF2B) ELISA Kit employs a two-site sandwich ELISA to quantitate OTF2B in samples. An antibody specific for OTF2B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyOTF2B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for OTF2B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of OTF2B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Octamer transcription factor 2B microplate
• Human Octamer transcription factor 2B standard
• Human Octamer transcription factor 2B detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Octamer transcription factor 2B (OTF2B) ELISA Kit has high sensitivity and excellent specificity for detection of Human OTF2B. No significant cross-reactivity or interference between Human OTF2B and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Glucocorticoid receptor (GR) and octamer transcription factors 1 and 2 (Oct-1/2) interact synergistically to activate the transcription of mouse mammary tumor virus and many cellular genes. Synergism correlates with cooperative DNA binding of the two factors in vitro.

Alternative Names: OTF2B

Search name: OTF2B

Tag: OTF2B