Human OGT (O-Linked-N-Acetylglucosamine Transferase) ELISA Kit | ELK5156

Human OGT (O-Linked-N-Acetylglucosamine Transferase) ELISA Kit | ELK5156 | Gentaur UK, US & Europe Distribution

Alternative Names: HRNT1; O-GLCNAC; UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit; O-GlcNAc transferase subunit p110

Species: Human

Assay Type: Sandwich

Sensitivity: 0.59 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to O-Linked-N-Acetylglucosamine Transferase (OGT) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to O-Linked-N-Acetylglucosamine Transferase (OGT) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain O-Linked-N-Acetylglucosamine Transferase (OGT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of O-Linked-N-Acetylglucosamine Transferase (OGT) in the samples is then determined by comparing the OD of the samples to the standard curve.