Human P4Ha3 (Prolyl-4-Hydroxylase Alpha Polypeptide III) ELISA Kit | ELK3202

Human P4Ha3 (Prolyl-4-Hydroxylase Alpha Polypeptide III) ELISA Kit | ELK3202 | Gentaur UK, US & Europe Distribution

Alternative Names: Procollagen-Proline, 2-Oxoglutarate 4-Dioxygenase (Proline 4-Hydroxylase), Alpha Polypeptide III

Species: Human

Assay Type: Sandwich

Sensitivity: 0.066 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prolyl-4-Hydroxylase Alpha Polypeptide III (P4Ha3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Prolyl-4-Hydroxylase Alpha Polypeptide III (P4Ha3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prolyl-4-Hydroxylase Alpha Polypeptide III (P4Ha3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prolyl-4-Hydroxylase Alpha Polypeptide III (P4Ha3) in the samples is then determined by comparing the OD of the samples to the standard curve.