Human PADI6 (Peptidyl Arginine Deiminase Type VI) ELISA Kit | ELK3870

Human PADI6 (Peptidyl Arginine Deiminase Type VI) ELISA Kit | ELK3870 | Gentaur UK, US & Europe Distribution

Alternative Names: Peptidylarginine deiminase VI; Peptidyl arginine deiminase-like protein

Species: Human

Assay Type: Sandwich

Sensitivity: 0.062 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peptidyl Arginine Deiminase Type VI (PADI6) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Peptidyl Arginine Deiminase Type VI (PADI6) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peptidyl Arginine Deiminase Type VI (PADI6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Peptidyl Arginine Deiminase Type VI (PADI6) in the samples is then determined by comparing the OD of the samples to the standard curve.