Human PAEP (Progestagen Associated Endometrial Protein) ELISA Kit | ELK3531

Human PAEP (Progestagen Associated Endometrial Protein) ELISA Kit | ELK3531 | Gentaur UK, US & Europe Distribution

Alternative Names: GD; GdA; GdF; GdS; PAEG; PEP; PP14; Glycodelin; Placental Protein 14; Alpha Uterine Protein; Pregnancy-Associated Endometrial Alpha-2-Globulin; Progesterone-associated

Species: Human

Assay Type: Sandwich

Sensitivity: 2.1 ng/mL

Standard: 300 ng/mL

Detection Range: 4.69-300 ng/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Reproductive science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Progestagen Associated Endometrial Protein (PAEP) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Progestagen Associated Endometrial Protein (PAEP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Progestagen Associated Endometrial Protein (PAEP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Progestagen Associated Endometrial Protein (PAEP) in the samples is then determined by comparing the OD of the samples to the standard curve.