Human PAFAH2 (Platelet Activating Factor Acetylhydrolase 2) ELISA Kit | ELK1780

Human PAFAH2 (Platelet Activating Factor Acetylhydrolase 2) ELISA Kit | ELK1780 | Gentaur UK, US & Europe Distribution

Alternative Names: SD-PLA2; Serine-dependent phospholipase A2; Platelet-activating factor acetylhydrolase 2, cytoplasmic

Species: Human

Assay Type: Sandwich

Sensitivity: 0.105 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Plasma.

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Metabolic pathway;Hematology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Platelet Activating Factor Acetylhydrolase 2 (PAFAH2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Platelet Activating Factor Acetylhydrolase 2 (PAFAH2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Platelet Activating Factor Acetylhydrolase 2 (PAFAH2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Platelet Activating Factor Acetylhydrolase 2 (PAFAH2) in the samples is then determined by comparing the OD of the samples to the standard curve.