Human PAPPA (Pregnancy Associated Plasma Protein A) ELISA Kit | ELK1436

Human PAPPA (Pregnancy Associated Plasma Protein A) ELISA Kit | ELK1436 | Gentaur UK, US & Europe Distribution

Alternative Names: PAPP-A; PAPA; ASBABP2; DIPLA1; IGFBP-4ase; PAPPA1; Pappalysin 1; Insulin-Like Growth Factor Binding Protein-4 Protease; Differentially Placenta 1 Expressed Protein

Species: Human

Assay Type: Sandwich

Sensitivity: 0.117 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Reproductive science;Genetic science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pregnancy Associated Plasma Protein A (PAPPA) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pregnancy Associated Plasma Protein A (PAPPA) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pregnancy Associated Plasma Protein A (PAPPA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pregnancy Associated Plasma Protein A (PAPPA) in the samples is then determined by comparing the OD of the samples to the standard curve.