Human PC4 and SFRS1 interacting protein 1 (PSIP1) ELISA Kit | AE25528HU

Human PC4 and SFRS1 interacting protein 1 (PSIP1) ELISA Kit | AE25528HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: PSIP1

Alternative Name: DFS70; LEDGF; MGC74712; PAIP; PSIP2; p52; p75; transcriptional coactivator p52/p75

Application: ELISA

Range: 46.88-3000 pg/mL

Sensitivity: 11.72 pg/mL

Intra-Assay: ≤4.5%

Inter-Assay: ≤8.2%

Recovery: 1, 01

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate PSIP1 in samples. An antibody specific for PSIP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPSIP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PSIP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PSIP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: PC4 and SFRS1 interacting protein 1, also known as lens epithelium-derived growth factor (LEDGF/p75), dense fine speckles 70kD protein (DFS 70) or transcriptional coactivator p75/p52, is a protein that in humans is encoded by the PSIP1 gene.PSIP1 has not been clearly linked to a specific cellular mechanism. The term LEDGF/p75 (Lens epithelium-derived growth factor) has entered common usage based on the initial characterization of PSIP1, however this is a misnomer, as the protein is present in most tissues and has no direct role in the development of lens epithelium. LEDGF/p75, a transcription coactivator, gained prominence as a host factor that assists HIV integration and is probably the only integrase interactor whose knock-down severely affects the HIV integration levels. The interaction between HIV integrase and human LEDGF/p75 is a promising target for anti-HIV drug discovery.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .