Human PCGF4 (Polycomb Group Ring Finger Protein 4) ELISA Kit | ELK3827

Human PCGF4 (Polycomb Group Ring Finger Protein 4) ELISA Kit | ELK3827 | Gentaur UK, US & Europe Distribution

Alternative Names: BMI1; RNF51; BMI1 Polycomb Ring Finger Oncogene; RING finger protein 51; Polycomb complex protein BMI-1

Species: Human

Assay Type: Sandwich

Sensitivity: 0.122 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Polycomb Group Ring Finger Protein 4 (PCGF4) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Polycomb Group Ring Finger Protein 4 (PCGF4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Polycomb Group Ring Finger Protein 4 (PCGF4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Polycomb Group Ring Finger Protein 4 (PCGF4) in the samples is then determined by comparing the OD of the samples to the standard curve.