Human PDGFD (Platelet Derived Growth Factor D) ELISA Kit | ELK2975

Human PDGFD (Platelet Derived Growth Factor D) ELISA Kit | ELK2975 | Gentaur UK, US & Europe Distribution

Alternative Names: PDGF-D; IEGF; SCDGF-B; Spinal Cord Derived Growth Factor B; Iris-expressed growth factor; Platelet-derived growth factor D, receptor-binding form/latent form

Species: Human

Assay Type: Sandwich

Sensitivity: 4.7 pg/mL

Standard: 800 pg/mL

Detection Range: 12.5-800 pg/mL

Sample Type: Serum, plasma and other biological fluids

Assay Lenght: 3.5h

Research Area: Cytokine

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Platelet Derived Growth Factor D (PDGFD) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Platelet Derived Growth Factor D (PDGFD) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Platelet Derived Growth Factor D (PDGFD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Platelet Derived Growth Factor D (PDGFD) in the samples is then determined by comparing the OD of the samples to the standard curve.