Human PDGFRa (Platelet Derived Growth Factor Receptor Alpha) ELISA Kit | ELK2768

Human PDGFRa (Platelet Derived Growth Factor Receptor Alpha) ELISA Kit | ELK2768 | Gentaur UK, US & Europe Distribution

Alternative Names: CD140a; PDGFR2; Rhe-PDGFRA; CD140 antigen-like family member A; Platelet-derived growth factor receptor 2; Alpha-type platelet-derived growth factor receptor

Species: Human

Assay Type: Sandwich

Sensitivity: 0.064 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction;Infection immunity;Hematology;Kidney biomarker

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Platelet Derived Growth Factor Receptor Alpha (PDGFRa) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Platelet Derived Growth Factor Receptor Alpha (PDGFRa) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Platelet Derived Growth Factor Receptor Alpha (PDGFRa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Platelet Derived Growth Factor Receptor Alpha (PDGFRa) in the samples is then determined by comparing the OD of the samples to the standard curve.