Human PDHa (Pyruvate Dehydrogenase Alpha) ELISA Kit | ELK1709

Human PDHa (Pyruvate Dehydrogenase Alpha) ELISA Kit | ELK1709 | Gentaur UK, US & Europe Distribution

Alternative Names: PDHCE1A; PHE1A; PHE1-A; PDHA1; PDH-A; Pyruvate Dehydrogenase (lipoamide) Alpha 1; Pyruvate Dehydrogenase E1 Component Subunit Alpha, Aomatic Form, Mitochondrial

Species: Human

Assay Type: Sandwich

Sensitivity: 0.063 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Metabolic pathway

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pyruvate Dehydrogenase Alpha (PDHa) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pyruvate Dehydrogenase Alpha (PDHa) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pyruvate Dehydrogenase Alpha (PDHa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pyruvate Dehydrogenase Alpha (PDHa) in the samples is then determined by comparing the OD of the samples to the standard curve.