Human PDIA5 (Protein Disulfide Isomerase A5) ELISA Kit | ELK3307

Human PDIA5 (Protein Disulfide Isomerase A5) ELISA Kit | ELK3307 | Gentaur UK, US & Europe Distribution

Alternative Names: PDI-A5; PDIR; Protein Disulfide Isomerase-Associated 5; Protein disulfide isomerase-related protein

Species: Human

Assay Type: Sandwich

Sensitivity: 0.058 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Disulfide Isomerase A5 (PDIA5) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Protein Disulfide Isomerase A5 (PDIA5) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Disulfide Isomerase A5 (PDIA5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Disulfide Isomerase A5 (PDIA5) in the samples is then determined by comparing the OD of the samples to the standard curve.