Human PDK1 (Pyruvate Dehydrogenase Kinase Isozyme 1) ELISA Kit | ELK9121

Human PDK1 (Pyruvate Dehydrogenase Kinase Isozyme 1) ELISA Kit | ELK9121 | Gentaur UK, US & Europe Distribution

Alternative Names: [Pyruvate dehydrogenase (acetyl-transferring) ] kinase isozyme 1, mitochondrial

Species: Human

Assay Type: Sandwich

Sensitivity: 0.58 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1) in the samples is then determined by comparing the OD of the samples to the standard curve.