Human Peptidase inhibitor 16 (PI16) ELISA Kit | KTE61219

Human Peptidase inhibitor 16 (PI16) ELISA Kit | KTE61219 | Gentaur UK, US & Europe Distribution

Application: This Human Peptidase inhibitor 16 (PI16) ELISA Kit employs a two-site sandwich ELISA to quantitate PI16 in samples. An antibody specific for PI16 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPI16 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PI16 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PI16 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Peptidase inhibitor 16 microplate
• Human Peptidase inhibitor 16 standard
• Human Peptidase inhibitor 16 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Peptidase inhibitor 16 (PI16) ELISA Kit has high sensitivity and excellent specificity for detection of Human PI16. No significant cross-reactivity or interference between Human PI16 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: PI16 is a putative serine protease inhibitor. PI16 may serve as a marker following prostatectomy for prostate cancer. This protein binds with high affinity to PSP94. PI16 expression is enhanced after serum stimulation of primary cardiomyocytes and is rapidly secreted into the conditioned medium of cardiomyocytes. PI16 is overexpressed in the hypertrophic and failing heart and inhibits cardiomyocyte growth. Suppression of endogenous PI16 expression in primary cardiomyocytes significantly enhances cardiomyocyte size. Transgenic mice overexpressing PI16 in a cardiomyocyte-specific manner show normal cardiac function but have smaller hearts with hypotrophic cardiomyocytes.

Alternative Names: PI16; CRISP9; DKFZp586B1817; MGC45378; MSMBBP; PSPBP; PSP94-binding protein; microseminoprotein; beta-binding protein; protease inhibitor 16

Search name: PI16; CRISP9; DKFZp586B1817; MGC45378; MSMBBP; PSPBP; PSP94-binding protein; microseminoprotein; beta-binding protein; protease inhibitor 16

Tag: PI16