Human Periostin (POSTN) ELISA Kit | AE26569HU

Human Periostin (POSTN) ELISA Kit | AE26569HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: POSTN

Alternative Name: MGC119510; MGC119511; OSF-2; OSF2; PDLPOSTN; PN; RP11-412K4.1; periostin; osteoblast specific factor 2 (fasciclin I-like) |periodontal ligament-specific periostin|periostin isoform thy2|periostin iso;

Application: ELISA

Range: 31.25-2000 ng/mL

Sensitivity: 14.5 ng/mL

Intra-Assay: ≤6.2%

Inter-Assay: ≤8.4%

Recovery: 1, 01

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate POSTN in samples. An antibody specific for POSTN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPOSTN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POSTN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POSTN bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: By screening human placenta and osteosarcoma cDNA libraries with mouse Postn as probe, they cloned 2 variants of human POSTN. One variant encodes a deduced 779-amino acid protein with an apparent molecular mass of 87.0 kD, and the other encodes a deduced 836-amino acid protein with an apparent molecular mass of 93.3 kD. POSTN contains a typical signal sequence, followed by a cysteine-rich domain, a 4-fold repeat structure of about 150 amino acids, and a C-terminal domain. Mouse and human POSTN share 89.2% amino acid identity overall and 90.1% identity in their mature forms. Northern blot analysis of a mouse osteoblastic cell line detected a 3.4-kb Postn transcript. RNA dot blot analysis detected expression of Postn in primary mouse osteoblasts and in lung, but not in any other tissues examined.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .