Human PGA (Pepsinogen A) ELISA Kit | ELK2325

Human PGA (Pepsinogen A) ELISA Kit | ELK2325 | Gentaur UK, US & Europe Distribution

Alternative Names: PGA5; PG-A; Pepsinogen 5 Group I; Pepsinogen-5; Pepsin A-5; Pepsinogen I

Species: Human

Assay Type: Sandwich

Sensitivity: 1.22 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Sample Type: Serum, plasma and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Tumor immunity;Gastroenterology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pepsinogen A (PGA) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pepsinogen A (PGA) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pepsinogen A (PGA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pepsinogen A (PGA) in the samples is then determined by comparing the OD of the samples to the standard curve.