Human Pgp (Permeability Glycoprotein) ELISA Kit | ELK2148

Human Pgp (Permeability Glycoprotein) ELISA Kit | ELK2148 | Gentaur UK, US & Europe Distribution

Alternative Names: CD243; ABCB1; ABC20; CLCS; GP170; MDR1; PGY1; P-Glycoprotein; Multidrug resistance protein 1; ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1; Colchicin Sensitivity

Species: Human

Assay Type: Sandwich

Sensitivity: 0.121 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Permeability Glycoprotein (Pgp) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Permeability Glycoprotein (Pgp) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Permeability Glycoprotein (Pgp), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Permeability Glycoprotein (Pgp) in the samples is then determined by comparing the OD of the samples to the standard curve.