Human Phosphoenolpyruvate carboxykinase [GTP], mitochondrial (PCK2) ELISA Kit | AE28414HU

Human Phosphoenolpyruvate carboxykinase [GTP], mitochondrial (PCK2) ELISA Kit | AE28414HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: PCK2

Alternative Name: PEPCK; PEPCK-M; PEPCK2; PEP carboxykinase|mitochondrial phosphoenolpyruvate carboxykinase 2|phosphoenolpyruvate carboxylase|phosphopyruvate carboxylase

Application: ELISA

Range: 1.56-100 ng/mL

Sensitivity: 0.74 ng/mL

Intra-Assay: ≤4.8%

Inter-Assay: ≤8.7%

Recovery: 1, 01

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate PCK2 in samples. An antibody specific for PCK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPCK2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCK2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCK2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Phosphoenolpyruvate Carboxykinase 2, Mitochondrial is a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described. PCK2 activation mediates an adaptive response to glucose depletion in lung cancer.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .