Human Phospholipase B1, membrane-associated (PLB1) ELISA Kit | KTE62208

Human Phospholipase B1, membrane-associated (PLB1) ELISA Kit | KTE62208 | Gentaur UK, US & Europe Distribution

Application: This Human Phospholipase B1, membrane-associated (PLB1) ELISA Kit employs a two-site sandwich ELISA to quantitate PLB1 in samples. An antibody specific for PLB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Phospholipase B1, membrane-associated microplate
• Human Phospholipase B1, membrane-associated standard
• Human Phospholipase B1, membrane-associated detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Phospholipase B1, membrane-associated (PLB1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PLB1. No significant cross-reactivity or interference between Human PLB1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: By Western blot analysis using an antibody against guinea pig phospholipase B (PLB), Maury et al. (2002) detected a 97-kD polypeptide in the soluble and membrane fractions of human epidermis that was enriched in the soluble fraction. Consistent with these results, they found that PLB activity was greatest in the soluble fraction. Trypsin had no effect on the molecular mass or enzymatic activity, suggesting that the protein they detected was already in an active form. Immunolocalization experiments revealed that PLB is expressed in the entire human epidermis with an accumulation in the dermoepidermis junction. In situ hybridization also indicated expression in the entire epidermis.

Alternative Names: PLB1; PLB; PLB/LIP; lysophospholipase; phospholipase A2

Search name: PLB1; PLB; PLB/LIP; lysophospholipase; phospholipase A2

Tag: PLB1