Human Placenta lactogen (HPL) ELISA Kit | KTE62417

Human Placenta lactogen (HPL) ELISA Kit | KTE62417 | Gentaur UK, US & Europe Distribution

Application: This Human Placenta lactogen (HPL) ELISA Kit employs a two-site sandwich ELISA to quantitate CSH1 in samples. An antibody specific for CSH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCSH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CSH1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CSH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Placenta lactogen microplate
• Human Placenta lactogen standard
• Human Placenta lactogen detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Placenta lactogen (HPL) ELISA Kit has high sensitivity and excellent specificity for detection of Human CSH1. No significant cross-reactivity or interference between Human CSH1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Human placental lactogen (HPL), also called human chorionic somatomammotropin, is a polypeptide placental hormone. Its structure and function is similar to that of human growth hormone. It modifies the metabolic state of the mother during pregnancy to facilitate the energy supply of the fetus. HPL is an anti-insulin.HPL consists of 190 amino acids that are linked by two disulfite bonds and is secreted by the syncytiotrophoblast during pregnancy. Its molecular weight is 22, 125. Like human growth hormone HPL is encoded by genes on chromosome 17q22-24. Its biologic half-life is 15 minutes.HPL is only present during pregnancy with maternal serum levels rising in relation to the growth of the fetus and placenta. Maximum levels are reached near term, typically to 5–7 mg/ml. Higher levels are noted in patients with multiple gestation.

Alternative Names: CSH1; CSA; CSMT; FLJ75407; PL; CS-1; choriomammotropin; chorionic somatomammotropin A; chorionic somatomammotropin hormone 1; hCS-A; placental lactogen

Search name: CSH1; CSA; CSMT; FLJ75407; PL; CS-1; choriomammotropin; chorionic somatomammotropin A; chorionic somatomammotropin hormone 1; hCS-A; placental lactogen

Tag: CSH1