Human Placental protein 13 (PP13) ELISA Kit | AE58412HU

Human Placental protein 13 (PP13) ELISA Kit | AE58412HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: LGALS13

Alternative Name: GAL13; PLAC8; PP13; beta-galactoside-binding lectin|galectin 13|galectin-13|placental protein 13

Application: ELISA

Range: 15.6-1000 pg/mL

Sensitivity: 6.3 pg/mL

Intra-Assay: ≤5.2%

Inter-Assay: ≤7.6%

Recovery: 0, 91

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate LGALS13 in samples. An antibody specific for LGALS13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLGALS13 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LGALS13 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LGALS13 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. Galactoside-binding soluble lectin 13 has lysophospholipase activity. It is composed of two identical subunits which are held together by disulfide bonds. This protein has structural similarity to several members of the beta-galactoside-binding S-type lectin family.By ultracentrifugation and SDS-PAGE, LGALS13 showed an apparent molecular mass of 29 to 30 kD. Under reducing conditions, the apparent molecular mass was 16 kD, suggesting that LGALS13 is composed of 2 identical subunits held together by disulfide bonds. Western blot analysis and Ouchterlony gel diffusion were unable to detect LGALS13 in serum or in any other adult or fetal tissue examined.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .