Human Plakophilin-1 (PKP1) ELISA Kit | KTE62209

Human Plakophilin-1 (PKP1) ELISA Kit | KTE62209 | Gentaur UK, US & Europe Distribution

Application: This Human Plakophilin-1 (PKP1) ELISA Kit employs a two-site sandwich ELISA to quantitate PKP1 in samples. An antibody specific for PKP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPKP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PKP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PKP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Plakophilin-1 microplate
• Human Plakophilin-1 standard
• Human Plakophilin-1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Plakophilin-1 (PKP1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PKP1. No significant cross-reactivity or interference between Human PKP1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Plakophilin-1 is a member of the arm-repeat (armadillo) and plakophilin gene families. Plakophilin proteins contain numerous armadillo repeats, localize to cell desmosomes and nuclei, and participate in linking cadherins to intermediate filaments in the cytoskeleton. This protein may be involved in molecular recruitment and stabilization during desmosome formation. Mutations in this gene have been associated with the ectodermal dysplasia/skin fragility syndrome.Compared with those in controls, desmosomes in PKP1-N/A patients were reduced dramatically both in size (49%) and frequency (61%) in the lower suprabasal layers (P less than 0.01) . In the lower suprabasal compartment of the heterozygous carrier, corresponding reductions were 37% and 20%, respectively (P less than 0.01) .

Alternative Names: PKP1; B6P; MGC138829; OTTHUMP00000033849; band 6 protein; plakophilin 1

Search name: PKP1; B6P; MGC138829; OTTHUMP00000033849; band 6 protein; plakophilin 1

Tag: PKP1