Human Pleiotrophin (PTN) ELISA Kit | KTE62598

Human Pleiotrophin (PTN) ELISA Kit | KTE62598 | Gentaur UK, US & Europe Distribution

Application: This Human Pleiotrophin (PTN) ELISA Kit employs a two-site sandwich ELISA to quantitate PTN in samples. An antibody specific for PTN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTN bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Pleiotrophin microplate
• Human Pleiotrophin standard
• Human Pleiotrophin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Pleiotrophin (PTN) ELISA Kit has high sensitivity and excellent specificity for detection of Human PTN. No significant cross-reactivity or interference between Human PTN and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Pleiotrophin was initially recognized as a neurite outgrowth-promoting factor present in rat brain around birth and as a mitogen toward fibroblasts isolated from bovine uterus tissue. Together with midkine these growth-factors constitute a family of secreted heparin-binding proteins now known as the Neurite Growth-promoting Factor (NEGF) family. During embryonic and early postnatal development, Pleiotrophin is expressed in the central and peripheral nervous system and also in several non-neural tissues, notably lung, kidney, gut and bone. Pleiotrophin is also expressed by several tumor cells and is thought to be involved in tumor angiogenesis. In the adult central nervous system.  Pleiotrophin is expressed in an activity-dependent manner in the hippocampus, where it can suppress long term potentiation induction.

Alternative Names: PTN; HARP; HBGF8; HBNF; NEGF1; heparin affin regulatory protein; heparin binding growth factor 8; heparin-binding growth-associated molecule; neurite growth-promoting factor 1; pleiotrophin (heparin binding

Search name: PTN; HARP; HBGF8; HBNF; NEGF1; heparin affin regulatory protein; heparin binding growth factor 8; heparin-binding growth-associated molecule; neurite growth-promoting factor 1; pleiotrophin (heparin binding

Tag: PTN