Human Plexin-B2 (PLXNB2) ELISA Kit | KTE61202

Human Plexin-B2 (PLXNB2) ELISA Kit | KTE61202 | Gentaur UK, US & Europe Distribution

Application: This Human Plexin-B2 (PLXNB2) ELISA Kit employs a two-site sandwich ELISA to quantitate PLXNB2 in samples. An antibody specific for PLXNB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLXNB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLXNB2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLXNB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Plexin-B2 microplate
• Human Plexin-B2 standard
• Human Plexin-B2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Plexin-B2 (PLXNB2) ELISA Kit has high sensitivity and excellent specificity for detection of Human PLXNB2. No significant cross-reactivity or interference between Human PLXNB2 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Members of the B class of plexins, such as PLXNB2 is related to human SEP (PLXNB1) . RT-PCR detected highest PLXNB2 expression in testis and ovary, followed by placenta, lung, liver, and kidney. Much lower expression was detected in prostate and small intestine, and little to none was detected in other tissues examined. Ligand-induced dimerization of plexin B was sufficient to stimulate endogenous RhoA (ARHA) potently and induce the reorganization of the cytoskeleton in mammalian cells. Overexpression of the PDZ domain of PDZ-RhoGEF prevented cell rounding and neurite retraction in differentiated rat neural precursor cells induced by activation of endogenous PLXNB1 by semaphorin-4D .

Alternative Names: PLXNB2; RP3-402G11.19; KIAA0315; MM1; Nbla00445; PLEXB2; dJ402G11.3; OTTHUMP00000196636

Search name: PLXNB2; RP3-402G11.19; KIAA0315; MM1; Nbla00445; PLEXB2; dJ402G11.3; OTTHUMP00000196636

Tag: PLXNB2