Human Plexin-D1 (PLXND1) ELISA Kit | KTE62357

Human Plexin-D1 (PLXND1) ELISA Kit | KTE62357 | Gentaur UK, US & Europe Distribution

Application: This Human Plexin-D1 (PLXND1) ELISA Kit employs a two-site sandwich ELISA to quantitate PLXND1 in samples. An antibody specific for PLXND1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLXND1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLXND1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLXND1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Plexin-D1 microplate
• Human Plexin-D1 standard
• Human Plexin-D1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Plexin-D1 (PLXND1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PLXND1. No significant cross-reactivity or interference between Human PLXND1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Tamagnone et al. (1999) identified the cDNA sequence of a novel member of the plexin gene family and named the gene plexin D1. Plxnd1 was expressed by embryonic vascular endothelial cells. Expression was robust in endocardium, intersomitic vessels, pulmonary vasculature, aorta, and pharyngeal arch arteries. In adult mice, Plxnd1 was expressed in heart, brain, lung, kidney, and testis, consistent with broad expression in endothelial cells. Human vascular endothelial cells also expressed PLXND1. Gitler et al. (2004) demonstrated that mouse Plxnd1 heterodimerized with Npn1 to form a functional Sema3C receptor. Binding of Sema3A and Sema3C to COS cells expressing Npn1 was enhanced by cotransfection of Plxnd1. Plxnd1 also enhanced binding of Sema3C to Npn2.

Alternative Names: PLXND1; KIAA0620; MGC75353; PLEXD1;

Search name: PLXND1; KIAA0620; MGC75353; PLEXD1;

Tag: PLXND1