Human PLP2 (Proteolipid Protein 2, Colonic Epithelium Enriched) ELISA Kit | ELK4784

Human PLP2 (Proteolipid Protein 2, Colonic Epithelium Enriched) ELISA Kit | ELK4784 | Gentaur UK, US & Europe Distribution

Alternative Names: A4; A4-LSB; A4 Differentiation-Dependent Protein; Intestinal membrane A4 protein

Species: Human

Assay Type: Sandwich

Sensitivity: 0.065 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Metabolic pathway

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proteolipid Protein 2, Colonic Epithelium Enriched (PLP2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Proteolipid Protein 2, Colonic Epithelium Enriched (PLP2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Proteolipid Protein 2, Colonic Epithelium Enriched (PLP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Proteolipid Protein 2, Colonic Epithelium Enriched (PLP2) in the samples is then determined by comparing the OD of the samples to the standard curve.