Human POLb (Polymerase DNA Directed Beta) ELISA Kit | ELK4987

Human POLb (Polymerase DNA Directed Beta) ELISA Kit | ELK4987 | Gentaur UK, US & Europe Distribution

Alternative Names: Pol B; Pol beta; POLB

Species: Human

Assay Type: Sandwich

Sensitivity: 0.051 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Polymerase DNA Directed Beta (POLb) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Polymerase DNA Directed Beta (POLb) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Polymerase DNA Directed Beta (POLb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Polymerase DNA Directed Beta (POLb) in the samples is then determined by comparing the OD of the samples to the standard curve.