Human Poliovirus receptor (PVR) ELISA Kit | AE24973HU

Human Poliovirus receptor (PVR) ELISA Kit | AE24973HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: PVR

Alternative Name: CD155; FLJ25946; HVED; NECL5; Necl-5; PVS; TAGE4; nectin-like 5

Application: ELISA

Range: 15.6-1000 pg/mL

Sensitivity: 6.0 pg/mL

Intra-Assay: ≤4.8%

Inter-Assay: ≤8.4%

Recovery: 1, 04

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate PVR in samples. An antibody specific for PVR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPVR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PVR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PVR bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: CD155 is a Type I transmembrane glycoprotein in the immunoglobulin superfamily. Commonly known as Poliovirus Receptor (PVR) due to its involvement in the cellular poliovirus infection in primates, CD155's normal cellular function is in the establishment of intercellular adherens junctions between epithelial cells.The role of CD155 in the immune system is unclear, though it may be involved in intestinal humoral immune responses. This transfer is more important in some tumor cells expressing a lot of PVR, and may trigger fratricide NK cell activation, providing tumors with a mechanism of immunoevasion. Plays a role in mediating tumor cell invasion and migration. Serves as a receptor for poliovirus attachment to target cells.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .