Human Poliovirus receptor-related protein 3 (PVRL3) ELISA Kit | AE24965HU

Human Poliovirus receptor-related protein 3 (PVRL3) ELISA Kit | AE24965HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: PVRL3

Alternative Name: CD113; CDw113; DKFZp566B0846; FLJ90624; PPR3; PRR3; PVRR3; nectin-3; nectin 3

Application: ELISA

Range: 0.156-10 ng/mL

Sensitivity: 0.1 ng/mL

Intra-Assay: ≤5.7%

Inter-Assay: ≤8.3%

Recovery: 1, 01

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate PVRL3 in samples. An antibody specific for PVRL3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPVRL3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PVRL3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PVRL3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Reymond et al. (2000) cloned a full-length cDNA encoding PRR3. The deduced 550-amino acid PRR3 protein has a calculated molecular mass of 55.4 kD and contains an N-terminal hydrophobic leader sequence; an ectodomain containing a V-type Ig domain and 2 C-type Ig domains bracketed by 6 cysteine residues; a hydrophobic transmembrane region; and a long cytoplasmic sequence. PRR3 also has 6 potential N-glycosylation sites. Northern blot analysis revealed PRR3 expression predominantly in placenta and testis, with faint or no expression in other tissues. A major transcript of 2.8 kb and minor transcripts of 9.5, 6.0, and 4.7 kb were detected in placenta. PRR3 was also expressed in mammalian epithelial cell lines. Flow cytometry showed expression of PRR3 at the surface of transfected COS-1 cells.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .