Human Polyadenylate-binding protein 1-like (PABPC1L) ELISA Kit | AE28857HU

Human Polyadenylate-binding protein 1-like (PABPC1L) ELISA Kit | AE28857HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: PABPC1L

Alternative Name: RP5-1069P2.6; C20orf119; FLJ11840; FLJ30809; FLJ42053; PABPC1L1; dJ1069P2.3; ePAB; OTTHUMP00000063224|poly (A) -binding protein; cytoplasmic 1-like

Application: ELISA

Range: 0.312-20 ng/mL

Sensitivity: 0.115 ng/mL

Intra-Assay: ≤4.9%

Inter-Assay: ≤8.5%

Recovery: 1, 05

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate PABPC1L in samples. An antibody specific for PABPC1L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPABPC1L present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PABPC1L is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PABPC1L bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: PABPC1L belongs to the polyadenylate-binding protein type-1 family. Recent linkage studies and association analyses indicate the presence of at least one type 2 diabetes susceptibility gene in human chromosome region 20q12-q13.1. The map currently provides near-continuous coverage between SGC32867 and WI-17676 . EST database searches and genomic sequence alignments of ESTs, mRNAs, and UniGene clusters enabled the annotation of the sequence interval with experimentally confirmed and putative transcripts. The combination of the BAC transcript map, YAC-to-BAC scaffold, and reference Human Genome Project sequence provides a powerful integrated resource for future genomic analysis of this region.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .