Human POTEG (POTE Ankyrin Domain Family, Member G) ELISA Kit | ELK6576

Human POTEG (POTE Ankyrin Domain Family, Member G) ELISA Kit | ELK6576 | Gentaur UK, US & Europe Distribution

Alternative Names: A26C2; POTE14; POTE14a; CT104.4; ANKRD26-Like Family C Member 2; Cancer/Testis Antigen Family 104, Member 4; Prostate, ovary, testis-expressed protein on chromosome 14

Species: Human

Assay Type: Sandwich

Sensitivity: 0.117 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Tumor immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to POTE Ankyrin Domain Family, Member G (POTEG) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to POTE Ankyrin Domain Family, Member G (POTEG) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain POTE Ankyrin Domain Family, Member G (POTEG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of POTE Ankyrin Domain Family, Member G (POTEG) in the samples is then determined by comparing the OD of the samples to the standard curve.