Human PP (Pancreatic Polypeptide) ELISA Kit | ELK1833

Human PP (Pancreatic Polypeptide) ELISA Kit | ELK1833 | Gentaur UK, US & Europe Distribution

Alternative Names: PPY; PNP; Pancreatic Polypeptide Y; Obinepitide; Pancreatic icosapeptide; Pancreatic prohormone

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 14.2 pg/mL

Standard: 3000 pg/mL

Detection Range: 46.88-3000 pg/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 2h

Research Area: Endocrinology

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Pancreatic Polypeptide (PP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pancreatic Polypeptide (PP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pancreatic Polypeptide (PP) in the samples is then determined by comparing the OD of the samples to the standard curve.