Human PP (Pepsin) ELISA Kit | ELK8433

Human PP (Pepsin) ELISA Kit | ELK8433 | Gentaur UK, US & Europe Distribution

Alternative Names: PP; Pepsin

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 0.93 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Sample Type: Serum, plasma and other biological fluids

Assay Lenght: 2h

Research Area: Enzyme & Kinase;Tumor immunity;Gastroenterology

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Pepsin (PP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pepsin (PP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pepsin (PP) in the samples is then determined by comparing the OD of the samples to the standard curve.