Human PR3 (Proteinase 3) ELISA Kit | ELK3758

Human PR3 (Proteinase 3) ELISA Kit | ELK3758 | Gentaur UK, US & Europe Distribution

Alternative Names: MBN; PRTN3; ACPA; AGP7; C-ANCA; MBT; P29; PRK; Myeloblastin; Serine Proteinase, Neutrophil, Wegener Granulomatosis Autoantigen; Protease K; Proteinase K; Endopeptidase K

Species: Human

Assay Type: Sandwich

Sensitivity: 0.061 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Infection immunity;Autoimmunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proteinase 3 (PR3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Proteinase 3 (PR3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Proteinase 3 (PR3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Proteinase 3 (PR3) in the samples is then determined by comparing the OD of the samples to the standard curve.