Human PRB1 (Basic Salivary Proline Rich Protein 1) ELISA Kit | ELK3536

Human PRB1 (Basic Salivary Proline Rich Protein 1) ELISA Kit | ELK3536 | Gentaur UK, US & Europe Distribution

Alternative Names: PM; PMF; PMS; PRB1L; PRB1M; Ps 1; Ps 2; PRPPRB1; Ps; cP7; Proline-Rich Protein BstNI Subfamily 1

Species: Human

Assay Type: Sandwich

Sensitivity: 0.59 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: saliva and other biological fluids

Assay Lenght: 3.5h

Research Area: Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Basic Salivary Proline Rich Protein 1 (PRB1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Basic Salivary Proline Rich Protein 1 (PRB1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Basic Salivary Proline Rich Protein 1 (PRB1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Basic Salivary Proline Rich Protein 1 (PRB1) in the samples is then determined by comparing the OD of the samples to the standard curve.