Human PRDX5 (Peroxiredoxin 5) ELISA Kit | ELK3019

Human PRDX5 (Peroxiredoxin 5) ELISA Kit | ELK3019 | Gentaur UK, US & Europe Distribution

Alternative Names: PLP; PRDX6; ACR1; PMP20; PRXV; SBBI10; Thioredoxin Peroxidase PMP20; Peroxisomal Antioxidant Enzyme; TPx Type VI; Liver Tissue 2D-Page Spot 71B; Alu Co-Repressor 1

Species: Human

Assay Type: Sandwich

Sensitivity: 12.8 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: Tissue homogenates, cerebrospinal fluid and other biological fluids.

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peroxiredoxin 5 (PRDX5) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Peroxiredoxin 5 (PRDX5) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peroxiredoxin 5 (PRDX5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Peroxiredoxin 5 (PRDX5) in the samples is then determined by comparing the OD of the samples to the standard curve.