Human PRG4 (Proteoglycan 4) ELISA Kit | ELK3428

Human PRG4 (Proteoglycan 4) ELISA Kit | ELK3428 | Gentaur UK, US & Europe Distribution

Alternative Names: MSF; CACP; HAPO; JCAP; SZP; Megakaryocyte Stimulating factor; Articular Superficial Zone Protein; Camptodactyly; Arthropathy, Coxa Vara, Pericarditis Syndrome

Species: Human

Assay Type: Sandwich

Sensitivity: 25.5 pg/mL

Standard: 4000 pg/mL

Detection Range: 62.5-4000 pg/mL

Sample Type: serum, plasma, synovial fluid and other biological fluids

Assay Lenght: 3.5h

Research Area: Metabolic pathway

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proteoglycan 4 (PRG4) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Proteoglycan 4 (PRG4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Proteoglycan 4 (PRG4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Proteoglycan 4 (PRG4) in the samples is then determined by comparing the OD of the samples to the standard curve.