Human Profilin-1 (PFN1) ELISA Kit | AE27879HU

Human Profilin-1 (PFN1) ELISA Kit | AE27879HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: PFN1

Alternative Name: N/A

Application: ELISA

Range: 78-5000 pg/mL

Sensitivity: 30 pg/mL

Intra-Assay: ≤4.9%

Inter-Assay: ≤8.5%

Recovery: 0, 8

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate PFN1 in samples. An antibody specific for PFN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPFN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PFN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PFN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Profilin-1 is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is thought to regulate actin polymerization in response to extracellular signals. Deletion of this gene is associated with Miller-Dieker syndrome. Profilin is a ubiquitous 12- to 15-kD protein which inhibits the polymerization of actin. It is thought to function by complexing with unpolymerized actin in vivo. Dissociation of the profilin-actin complex is caused by binding of phosphatidylinositol 4, 5-bisphosphate to profilin. Ampe et al. (1988) described the amino acid sequence of human platelet profilin and found it to have 95% homology to the sequence of calf spleen profilin.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .