Human Proline dehydrogenase 1, mitochondrial (PRODH) ELISA Kit | KTE61134

Human Proline dehydrogenase 1, mitochondrial (PRODH) ELISA Kit | KTE61134 | Gentaur UK, US & Europe Distribution

Application: This Human Proline dehydrogenase 1, mitochondrial (PRODH) ELISA Kit employs a two-site sandwich ELISA to quantitate PRODH in samples. An antibody specific for PRODH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRODH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRODH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRODH bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Proline dehydrogenase 1, mitochondrial microplate
• Human Proline dehydrogenase 1, mitochondrial standard
• Human Proline dehydrogenase 1, mitochondrial detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Proline dehydrogenase 1, mitochondrial (PRODH) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRODH. No significant cross-reactivity or interference between Human PRODH and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: PRODH encodes a mitochondrial protein that catalyzes the first step in proline degradation. Mutations in PRODH are associated with hyperprolinemia type 1 and susceptibility to schizophrenia 4 (SCZD4) . PRODH is located on chromosome 22q11.21, a region which has also been associated with the contiguous gene deletion syndromes, DiGeorge and CATCH22. Alternatively spliced transcript variants encoding different isoforms have been found for PRODH. 

Alternative Names: PRODH; FLJ33744; HSPOX2; MGC148078; MGC148079; PIG6; POX; PRODH1; PRODH2; SCZD4; TP53I6; proline dehydrogenase 1; proline oxidase 2; proline oxidase; mitochondrial; tumor protein p53 inducible protein 6

Search name: PRODH; FLJ33744; HSPOX2; MGC148078; MGC148079; PIG6; POX; PRODH1; PRODH2; SCZD4; TP53I6; proline dehydrogenase 1; proline oxidase 2; proline oxidase; mitochondrial; tumor protein p53 inducible protein 6

Tag: PRODH